Method of constructing transgenic bird using lentivirus...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/867 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2536599

It is intended to provide a lentivirus vector containing VSV-G in the coat protein; a recombinant bird host obtained by infecting a bird host with the lentivirus vector and a method of constructing the same; and a transgenic chimeric bird constructed by the infection with the lentivirus vector and its offspring. It is also intended to provide a method of constructing a G0 transgenic chimeric bird and its offspring which comprises infecting an early embryo in a fertilized bird egg with a lentivirus vector containing VSV-G in the coat protein and then hatching the embryo; and a G0 transgenic chimeric bird and its offspring constructed by the above method. Namely, a transgenic bird which is constructed by infecting an early bird embryo with a lentivirus vector having VSV-G in its coat protein.

La présente invention porte sur un vecteur lentivirus contenant un VSV-G dans la protéine de coque ; sur un oiseau hôte recombiné que l'on obtient en infectant un oiseau hôte avec le vecteur lentivirus et sur un procédé de construction de ce dernier ; sur un oiseau chimère transgénique que l'on construit par infection par le vecteur lentivirus précité, et sur sa descendance. L'invention se rapporte également à un procédé qui permet de construire un oiseau chimère transgénique G0 et sa descendance, selon lequel on infecte un embryon précoce dans un oeuf d'oiseau fertilisé avec un vecteur lentivirus contenant le VSV-G dans la protéine de coque et on laisse éclore l'embryon ; et à un oiseau chimère transgénique G0 et sa descendance, construits selon le procédé précité. L'invention concerne en particulier un oiseau transgénique que l'on construit en infectant un embryon précoce d'oiseau avec un vecteur lentivirus contenant un VSV-G dans la protéine de coque.

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