Method of controlling a cellular process in a multi-cellular...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2497863

A method of controlling a genetically-modified multi-cellular organism or a part thereof, comprising the following steps: (a) providing a multi-cellular organism or a part thereof, whereby cells of said multi-cellular organism or said part contain a heterologous nucleic acid, (b) causing expression of a I protein from said heterologous nucleic acid in at least some of said cells, wherein said protein is capable of (i) leaving a cell and entering other cells of said multi-cellular organism or a part thereof, (ii) causing expression of said protein in cells containing said heterologous nucleic acid, and optionally (iii) controlling a cellular process of interest.

Cette invention se rapporte à un procédé servant à réguler un organisme multicellulaire génétiquement modifié ou une partie de celui-ci et comprenant à cet effet les étapes suivantes: (a) former un organisme multicellulaire ou une partie de celui-ci, dont des cellules contiennent un acide nucléique hétérologue, (b) produire l'expression d'une protéine I à partir de cet acide nucléique hétérologue dans certaines au moins de ces cellules, cette protéine étant capable (i) de quitter une cellule et de pénétrer dans d'autres cellules de l'organisme multicellulaire ou d'une partie de celui-ci, (ii) produire l'expression de cette protéine dans des cellules contenant ledit acide nucléique hétérologue, et éventuellement (iii) réguler le processus cellulaire recherché.

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