C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) C07K 14/245 (2006.01) C07K 14/65 (2006.01) C12N 1/21 (2006.01) C12N 1/38 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2145388
Nucleic acid is provided encoding a molecule having certain variations within the phosphate-binding region of native E. col pstS. Additionally provided are bacterial cells comprising this nucleic acid under control of the native pstS gene promoter, and optionally further comprising nucleic acid encoding a polypeptide of interest under control of the alkaline phosphatase (AP) promoter. Bacterial cells containing both pstS variant nucleic acid and polypeptide nucleic acid are cultured in a medium at a concentration of inorganic phosphate that at all phases of cell growth is above the level at which the cells are starved for phosphate. Alternatively, the cells are cultured under conditions whereby the concentration of inorganic phosphate in the culture medium is controlled during the production period so that the polypeptide is produced under the control of the partially induced AP promoter.
Bass Steven
Swartz James R.
Dennison Associates
Genentech Inc.
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