Method of controlling the regioselectivity of glycosidic bonds

Details Method of controlling the regioselectivity of glycosidic bonds Method of controlling the regioselectivity of glycosidic bonds

C - Chemistry, Metallurgy

12

P

195/139

C12P 19/44 (2006.01) C07H 3/06 (2006.01) C12P 19/04 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01) C12P 19/18 (2006.01)

Patent

CA 1339112

A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the carbohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligo-saccharide or a saccharide analog and at least one aglycon which is C-, N-, C- or S-glycosidically bonded in 1-position, the .alpha.- or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligo-saccharide compound being separated from the reaction mixture.

524898

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