Method of counting leukocytes and leukocyte counter

G - Physics – 01 – N

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G01N 33/49 (2006.01) G01N 1/30 (2006.01) G01N 15/14 (2006.01) G01N 15/00 (2006.01)

Patent

CA 2309281

A leukocyte counting method which comprises adding a cytolytic agent to a platelet preparation solution or an erythrocyte preparation solution to solubilize the platelets or erythrocytes in the respective solution in a leukocyte container which comprises an opening section, a side section and a bottom section and wherein the whole or part of the side section is provided with a portion having a horizontal cross section progressively increasing in the direction from the bottom section toward the opening section, centrifuging the container to accumulate the leukocytes in the bottom section, and counting the leukocytes thus accumulated.

L'invention concerne un procédé de numération des globules blancs, qui consiste à ajouter un agent cytolytique à une solution de préparation de plaquettes ou à une solution de préparation de globules rouges, afin de solubiliser les plaquettes ou les globules rouges dans leurs solutions respectives, lesquels se trouvent à l'intérieur d'un récipient contenant des globules blancs. Ce récipient comprend une section ouverture, une section latérale, et une section inférieure, la totalité ou une partie de ladite section latérale présentant une coupe transversale horizontale, qui s'élargit progressivement dans le sens de ladite section inférieure, vers ladite section ouverture. Ce procédé consiste enfin à soumettre ledit récipient à une centrifugation, de sorte que les globules blancs s'accumulent dans ladite section inférieure, où l'on peut alors procéder à leur numération.

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