Method of coupling ligands to a solid phase in acidic...

Details Method of coupling ligands to a solid phase in acidic... Method of coupling ligands to a solid phase in acidic...

G - Physics

01

N

G01N 33/543 (2006.01) C07K 17/00 (2006.01)

Patent

CA 2274956

The present invention relates to a method for binding antibody or antigen molecules to solid phase. The reactive compound comprising an antibody or antigen molecule and a solide phase are formed by coupling a specific antibody or antigen molecule to a solid phase using a two-step reaction. The coupling is covalent, between the active groups of the solid phase and the antibody, a fragment thereof or the antigen molecule, in the presence of an anionic surfactant, after addding an alcaline/solution to raise the pH to the basic region.

L'invention concerne une méthode permettant de fixer des molécules anticorps ou antigènes sur une phase solide. On forme le composé réactif, qui comprend une molécule anticorps ou antigène et une phase solide, en couplant une molécule anticorps ou antigène spécifique à une phase solide par une réaction en deux étapes. Le couplage est covalent, entre les groupes actifs de la phase solide et l'anticorps, un fragment de celui-ci ou la molécule antigène, en présence d'un tensioactif anionique, après adjonction d'une solution alcaline pour faire monter le pH vers la zone basique.

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