Method of cultivating cell or tissue

Details Method of cultivating cell or tissue Method of cultivating cell or tissue

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12N 5/07 (2010.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12N 1/00 (2006.01)

Patent

CA 2656876

A method of cultivating a culture of cell, tissue, etc. There is provided a method of cultivating a culture containing a cell line or tissue (cell constitution (2)), including imparting bending motion to the culture. By virtue of applying bending force to a culture of cell, tissue, etc. (cell constitution (2)) to thereby curve the culture, continuous compression and extension in the direction of thickness from a concave portion toward convex portion thereof are induced so that physical stimulation and deformation not attained by conventional pressurization, shear and tension can be loaded on the culture to thereby realize the culture appropriate for restoration of the tissue at site accompanied by bending.

L'invention a pour objet un procédé de culture cellulaire, tissulaire, etc. contenant une lignée cellulaire ou un tissu (constitution cellulaire (2)), consistant à imprimer un mouvement de flexion à la culture. Par l'application d'une force de flexion à une culture cellulaire, tissulaire, etc. (constitution cellulaire (2)) afin de la courber, on induit une compression continue et une extension dans le sens de l'épaisseur d'une partie concave vers une partie convexe de sorte que la stimulation physique et la déformation, non touchées par la pressurisation conventionnelle, le cisaillement et la tension, peuvent être appliquées sur la culture afin d'obtenir la culture adéquate pour une restauration tissulaire sur place en même temps que la flexion.

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