Method of cultivating living cells and bioreactor therefor

Details Method of cultivating living cells and bioreactor therefor Method of cultivating living cells and bioreactor therefor

C - Chemistry, Metallurgy

12

N

195/145, 195/45

C12N 1/00 (2006.01) C12M 1/04 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01)

Patent

CA 1321159

- 28 - ABSTRACT OF THE DISCLOSURE In a bioreactor, oxygen is supplied to a suspen- sion of living cells using a carrier liquid in which the oxygen is soluble. The carrier liquid is immiscible in the culture medium and has a specific gravity greater than that of the culture medium, so that when the oxygen bearing carrier liquid in injected into the culture medium, it will give up its oxygen and coalesce at the bottom of the reactor. The carrier liquid is oxygenated through a membrane to avoid contamination. The oxygenator may be temperature controlled to control the temperature of the carrier liquid and thus the culture medium. The concentration of carbon dioxide may also be controlled to ensure that it does not become excessive. The reactor may carry out either batch processes or continuous perfusion.

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