Method of culturing anchorage dependent cells

Details Method of culturing anchorage dependent cells Method of culturing anchorage dependent cells

C - Chemistry, Metallurgy

12

N

195/46

C12N 11/00 (2006.01) C07K 14/565 (2006.01) C12N 5/00 (2006.01) C12N 11/02 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1172586

METHOD OF CULTURING ANCHORAGE DEPENDENT CELLS Abstract of the Disclosure Disclosed is a method of growing anchorage-dependent cells: cells of the type which normally undergo mitosis only when anchored on a substrate, e.g., fibroblasts or epithelial cells. The method comprises the steps of encapsulating a seed culture of the cells within a semipermeable membrane and suspending the capsules in a growth medium. The interior sur- faces of the capsule membrane and/or collagen enclosed within the capsules serve as a substrate for the cells. The ratio of the available substrate surface area to the volume of the culture may be large, thereby allowing the cells to be grown substantially throughout the volume of the culture medium. -1-

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