Method of culturing mammalian respiratory epithelial cells

Details Method of culturing mammalian respiratory epithelial cells Method of culturing mammalian respiratory epithelial cells

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12M 3/04 (2006.01)

Patent

CA 2051993

A method of culturing respiratory epithelial cells in vitro on a fluid permeable collagen substrate derived from bovine chorionic membrane tissue. The epi- thelial cells are harvested from the nasal mucosa of a human donor using a minimally-invasive brushing technique. The culture method includes the step of incubating the plated cells in a relatively undisturbed state such that a substantial amount of the plated cells adhere, flatten and begin to replicate on the substrate surface.

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