Method of culturing protoplasts

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/02 (2006.01) A01H 4/00 (2006.01) C12M 3/04 (2006.01) C12N 5/04 (2006.01) C12N 5/14 (2006.01)

Patent

CA 1274201

ABSTRACT OF THE DISCLOSURE Disclosed is a method of culturing a protoplast in a liquid medium. According to the method of the present invention, the protoplast is cultured in a liquid medium layer of about 100 to about 400 µm thickness.

509544

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