C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/08 (2006.01) A61F 2/02 (2006.01) A61K 9/00 (2006.01) A61K 35/00 (2006.01) A61K 48/00 (2006.01) A61L 27/38 (2006.01) C08G 18/12 (2006.01) C08G 18/48 (2006.01) C08G 18/66 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2134088
2134088 9322427 PCTABS00027 A method of culturing live viable cells comprises culturing the cells under the conditions effective for cells to survive in the presence of a non-porous, semi-permeable biocompatible film of predefined characteristics having a tensile strength of 350-10,000 psi, an ultimate elongation of 300-1,500 %, a water absorption such that the sum of the volume fraction of absorbed water in the hydrophilic volume fraction of the soft segment is 100-2,000 % of the dried polymer volume and 50-95 % of the wet polymer volume. The film permeability can be changed to have different cut-off molecular weights while being substantially impermeable to cells and particulate matter as well as high molecular weight molecules. A method of regulating the level of a compound in a body fluid of the subject afflicted with an endogenous defect resulting in abnormal levels of the compound in the body fluid, in the substantial absence of a detrimental immunological reaction comprises: enclosing cells lacking the endogenous defect of the patient's cells in a biocompatible, implantable device, wherein at least one portion thereof comprises a non-porous, semi-permeable, biocompatible film substantially enclosing the cells, the film formed from a copolymer comprising about 5 to 45 wt% of at least one hard segment, and about 95 to 55 wt% of at least one soft segment comprising at least one hydrophilic, hydrophobic or amphipathic oligomer selected from the group consisting of aliphatic polyols, aliphatic and aromatic polyamines and mixtures thereof; the film having a tensile strength greater than about 350 psi and up to about 10,000 psi, an ultimate elongation greater than about 300 % and up to about 1,500 % and a water absorption such that the sum of the volume fraction of absorbed water and the hydrophilic volume fraction of the soft segment exceeds about 100 % and up to about 2,000 % of the dry polymer volume and exceeds about 50 % and up to about 95 % of the wet polymer volume, and the film being permeable to molecules of up to about 6,000 to 600,000 molecular weight and substantially impermeable to cells and particulate matter; implanting the device comprising the cells into a site in the subject's body where the cells are in contact with the subject's body fluid; and allowing the cells to grow at the implantation site where they are in direct interactive contact with the compound and act to regulate its level in the body fluid.
Kuhn Robert
Monahan John
Ward Robert S.
Polymer Technology Group Inc. (the)
Somatix Therapy Corporation
Swabey Ogilvy Renault
LandOfFree
Method of culturing viable cells and method of regulating... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of culturing viable cells and method of regulating..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of culturing viable cells and method of regulating... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1930077