Method of denaturing protein with enzymes

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 21/02 (2006.01) C12N 9/10 (2006.01) C12Q 1/48 (2006.01) C12Q 1/52 (2006.01)

Patent

CA 2717340

Provided are: a method of denaturing a protein by treating the protein with both of protein glutaminase and transglutaminase; a food containing a protein having been denatured with these enzymes; an enzyme preparation for denaturing a protein which comprises these enzymes; and a method of establishing the optimum quantitative ratio of protein glutaminase to transglutaminase both of which are to be added to a protein. A protein is denatured by adding protein glutaminase and transglutaminase to the protein substantially at the same timing, or adding protein glutaminase to the protein before the transglutaminase acts on the protein, or controlling the quantitative ratio of protein glutaminase to transglutaminase, by which a protein is treated, to a definite level. The optimum quantitative ratio of protein glutaminase to transglutaminase, both of which are to be added to a protein, is determined based on the NMR signal intensity of the protein labeled with isotope-labeled ammonium or the like.

La présente invention concerne : un procédé de dénaturation d'une protéine en traitant la protéine avec la protéine glutaminase et la transglutaminase ; un aliment contenant une protéine ayant été dénaturée avec ces enzymes ; une préparation d'enzymes pour dénaturer une protéine qui comprend ces enzymes ; et un procédé d'établissement du rapport quantitatif optimal de protéine glutaminase à la transglutaminase, toutes deux étant ajoutées à une protéine. Une protéine est dénaturée en ajoutant la protéine glutaminase et la transglutaminase à la protéine sensiblement en même temps, ou en ajoutant la protéine glutaminase à la protéine avant que la transglutaminase agisse sur la protéine, ou en contrôlant le rapport quantitatif de la protéine glutaminase à la transglutaminase, par lesquelles une protéine est traitée, à un niveau défini. Le rapport quantitatif optimal de la protéine glutaminase à la transglutaminase, toutes deux étant ajoutées à une protéine, est déterminé sur la base de l'intensité de signal RMN de la protéine marquée avec de l'ammonium marqué par un isotope ou similaire.

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