C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/67 (2006.01) C07K 14/445 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/30 (2006.01) C12P 21/02 (2006.01) G06F 17/30 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2480504
The present invention provides a method for modifying a wild type nucleic acid sequence encoding a polypeptide to enhance expression and accumulation of the polypeptide in the host cell by harmonizing synonymous codon usage frequency between the foreign DNA and the host cell DNA. This can be done by substituting codons in the foreign coding sequence with codons of similar usage frequency from the host DNA/RNA which code for the same amino acid. The present invention also provides novel synthetic nucleic acid sequences prepared by the method of the invention.
L'invention concerne un procédé pour modifier une séquence d'acide nucléique de type sauvage, codant un polypeptide, afin d'accroître l'expression et l'accumulation du polypeptide dans la cellule hôte par harmonisation de la fréquence d'utilisation de codons synonymes entre l'ADN étranger et l'ADN de la cellule hôte. A cet effet, ledit procédé consiste à substituer les codons dans la séquence de codage étrangère par des codons dont la fréquence d'utilisation est similaire, qui proviennent de l'ADN/ARN hôte et qui codent pour le même acide aminé. L'invention concerne également de nouvelles séquences d'acides nucléiques de synthèse préparées selon ledit procédé.
Angov Evelina
Kincaid Randall L.
Lyon Jeffrey A.
Borden Ladner Gervais Llp
Government Of The United States As Represented By The Secretary Of The Army
Veritas Inc.
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