Method of desulfurization of fossil fuel with flavoprotein

Details Method of desulfurization of fossil fuel with flavoprotein Method of desulfurization of fossil fuel with flavoprotein

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C10G 32/00 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/52 (2006.01) C12P 1/00 (2006.01) C12S 1/02 (2006.01)

Patent

CA 2206987

The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

Selon la présente invention, il est possible d'augmenter la vitesse de réaction de la désulfuration des combustibles fossiles par adjonction d'une flavoprotéine au biocatalyseur. La présente invention concerne un procédé augmentant la vitesse de désulfuration d'un combustible fossile contenant des composés soufrés organiques. Ce procédé consiste à: (a) mettre le combustible fossile en contact avec une phase aqueuse contenant un biocatalyseur capable de cliver les liaisons carbone-soufre et une quantité de flavoprotéine qui soit suffisante pour augmenter la vitesse de réaction, un mélange de combustible fossile et de phase aqueuse étant ainsi formé; (b) maintenir le mélange issu de l'opération (a) dans des conditions suffisantes pour que les liaisons carbone-soufre des molécules de soufrées organiques soient clivées par le biocatalyseur, un combustible fossile à teneur réduite en soufre organique étant ainsi formé; (c) séparer de la phase aqueuse résultante le combustible fossile à teneur réduite en soufre organique. L'invention concerne également un micro-organisme de recombinaison contenant une ou plusieurs molécules d'ADN de recombinaison codant un biocatalyseur capable de désulfurer un combustible fossile contenant des molécules de soufre organiques et codant une flavoprotéine. L'invention concerne enfin une composition constituée (a) d'un biocatalyseur capable de désulfurer un combustible fossile contenant des molécules de soufre organiques et (b) une flavoprotéine.

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