Method of detecting a specific microorganism

G - Physics – 01 – N

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167/45, 150/16.5

G01N 31/00 (2006.01) A01N 63/02 (2006.01) A61L 2/16 (2006.01) C12P 1/04 (2006.01) C12P 21/00 (2006.01) C12Q 1/18 (2006.01)

Patent

CA 1089748

METHOD OF DETECTING A SPECIFIC MICROORGANISM ABSTRACT OF THE DISCLOSURE A method of detecting a specific microorganism compris- ing contacting said microorganism with bacteriocins from a microorganism of a genus which is taxonomically unrelated to said specific organism. The result of such contact may be utilized to detect the presence of a micro- organism belonging to a taxonomically unrelated genus. Radio-labeled or fluorescein-labeled bacteriocins can be reacted with specific bacteria in a biological sample and the presence of such specific bacteria detected by remov- ing excess bacteriocins and determining the presence of fluorescent or radioactive bacteria in the sample. Neisseria gonorrhoeae is identified by spotting bacterio- cins on a plate of clinical material; or using a disk impregnated with bacteriocins placed on a plate inoculated with the clinical material; or the bacteriocins can be incorporated into one-half of a split agar plate, the identification being made on the basis of a zone of inhibition surrounding the spot where the bacteriocins were applied, or growth inhibition on the portion of the plate to which the bacteriocins were added.

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