G - Physics – 01 – N
Patent
G - Physics
01
N
167/45, 150/16.5
G01N 31/00 (2006.01) A01N 63/02 (2006.01) A61L 2/16 (2006.01) C12P 1/04 (2006.01) C12P 21/00 (2006.01) C12Q 1/18 (2006.01)
Patent
CA 1089748
METHOD OF DETECTING A SPECIFIC MICROORGANISM ABSTRACT OF THE DISCLOSURE A method of detecting a specific microorganism compris- ing contacting said microorganism with bacteriocins from a microorganism of a genus which is taxonomically unrelated to said specific organism. The result of such contact may be utilized to detect the presence of a micro- organism belonging to a taxonomically unrelated genus. Radio-labeled or fluorescein-labeled bacteriocins can be reacted with specific bacteria in a biological sample and the presence of such specific bacteria detected by remov- ing excess bacteriocins and determining the presence of fluorescent or radioactive bacteria in the sample. Neisseria gonorrhoeae is identified by spotting bacterio- cins on a plate of clinical material; or using a disk impregnated with bacteriocins placed on a plate inoculated with the clinical material; or the bacteriocins can be incorporated into one-half of a split agar plate, the identification being made on the basis of a zone of inhibition surrounding the spot where the bacteriocins were applied, or growth inhibition on the portion of the plate to which the bacteriocins were added.
274526
Iglewski Barbara H.
Morse Stephen A.
Oregon State Board Of Higher Education
Osler Hoskin & Harcourt Llp
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