Method of detecting a substance using enzymatically-induced...

Details Method of detecting a substance using enzymatically-induced... Method of detecting a substance using enzymatically-induced...

G - Physics

01

N

260/227, 167/45,

G01N 21/76 (2006.01) C07D 321/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1341031

In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula Image where T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; V is H or an enzyme-cleavable group; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzyme-cleavable group; and Z is H or an enzyme-cleavable group, provided that at least one of V, X, or Z must be an enzyme-cleavable group, so that the enzyme cleaves the enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane.

553645

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