Method of detecting analytes in a sample and support for...

G - Physics – 01 – N

Patent

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Details

G01N 33/551 (2006.01) B01J 19/00 (2006.01) G01N 33/543 (2006.01) G11B 7/013 (2006.01)

Patent

CA 2384337

The invention relates to a method of detecting analytes in a sample. According to said method, analyte-specific binders (15) are immobilized on a plurality of detection fields (5, 7) on one of the planar faces of a disk-shaped substrate (3). The sample is contacted with said detection fields (5, 7) and the presence or/and the quantity of the analytes (17) to be detected is determined by optically evaluating the detection fields (5, 7). According to the invention, a substrate (3) is used that is produced from an optically transparent material. The detection fields (5, 7) are arranged along at least one spiral line (27) distributed across the substrate (3). After the sample has been contacted with the detection fields (5, 7), a reflecting layer (21) is flatly applied on the substrate (3) on the planar face carrying the detection fields (5, 7). Alternatively, a magnetic read-out of the substrate can be made instead of the optical read-out.

L'invention concerne un procédé de détection d'analytes dans un échantillon pour essai, selon lequel sur un substrat en forme de disque (3), des liants spécifiques d'analytes (15) sont immobilisés, sur une de ses faces planes, en une pluralité de champs de détection (5, 7), l'échantillon pour essai est ensuite amené en contact avec les champs d'identification (5, 7), et l'on détermine la présence ou/et la quantité d'analytes à identifier (17) par évaluation optique des champs de détection (5, 7). L'invention est caractérisée en ce qu'on utilise un substrat (3) fabriqué en un matériau optiquement transparent, et en ce que les champs de détection (5, 7) répartis le long d'au moins une courbe en spirale (27) sont disposés sur le substrat (3). Après mise en contact de l'échantillon pour essai avec les champs de détection (5, 7), le substrat (3) est muni sur sa face plane porteuse des champs de détection (5, 7), d'une couche de réflexion (21). En variante, on peut également utiliser, à la place d'une séparation optique, une séparation magnétique du substrat.

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