Method of detecting and quantifying ligands in liquids

Details Method of detecting and quantifying ligands in liquids Method of detecting and quantifying ligands in liquids

G - Physics

01

N

167/43

G01N 33/542 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1297406

METHOD OF DETECTING AND QUANTIFYING LIGANDS IN LIQUIDS Abstract of the Disclosure A method of determining a ligand of interest in a liquid is described. The method, which makes it possible to detect and quantify a ligand in a liquid, makes use of a biotin-avidin system which can be used to carry out immunoassays in both heterogeneous and homogeneous format. The basic components used in the method are a biotin-labeled substance (which is biotin-lableled ligand or biotin-labeled specific binding partner), biotin- labeled fluorophore, a liquid to be analyzed for the ligand of interest, specific binding partner for the ligand of interest and avidin. The method can be used to determine the presence (or absence) and the quantity of avidin and biotin in a sample, using fluorescence polarization.

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