Method of detecting antigens or antibodies

G - Physics – 01 – N

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167/41, 167/43

G01N 23/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1036493

ABSTRACT OF THE DISCLOSURE This disclosure oncerns a solid phase method for de- tecting antigens or antibodies. More specifically there is disclosed a method for detecting the presence of proteins in samples, wherein said proteins are capable of acting as antigens or antibodies, said method comprising: coating at least a por- tion of the surface of a pair of receptacles, made of a water- insoluble material, with a known antigen; adding sample to said pair of receptacles; allowing said pair of receptacles to remain undisturbed for a predetermined period of time; adding labeled antibody to one of the receptacles of said pair; removing the contents of the other receptacle of said pair and then adding to said other receptacle an amount of labeled antibody equal to that added to said one receptacle in step (d); allowing said pair of receptacles to remain undisturbed for a predetermined period of time; removing the unbonded contents of each recep- tacle of said pair; measuring the amount of emitted radiation of each receptacle; comparing the amounts of emitted radiation of the two receptacles with the amounts of emitted radiation of negative controls to determine whether the sample contains either antigen or antibody or neither antigen nor antibody.

210357

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