C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01)
Patent
CA 2212193
The present invention provides an improved method of detecting or transferring a biological material whereby a biological material is adhered to a membrane which is then contacted with a detecting reagent, wherein the improvement comprises utilizing a polyvinylidene fluoride membrane which has been subjected to a temperature of at least about 80°C but less than the temperature at which the membrane softens and deforms for a time sufficient to reduce the ability of the detecting reagent to directly adhere to the membrane.
Méthode améliorée pour la détection ou le transfert d'une matière biologique, avec adhérence de cette matière à une membrane, qui est ensuite mise en contact avec un réactif détecteur; l'amélioration consiste à utiliser une membrane de poly(fluorure de vinylidène), soumise à une température d'au moins 80 degrés C environ, mais moins que la température à laquelle la membrane ramollit et se déforme suffisamment longtemps pour diminuer la capacité du réactif détecteur à y adhérer directement.
Marks & Clerk
Pall Corporation
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