Method of detecting blood constituent and kit therefor

Details Method of detecting blood constituent and kit therefor Method of detecting blood constituent and kit therefor

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/68 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2176150

A method for detecting blood component in a sample comprising reacting a human erythrocyte membrane band 3 glycoprotein (band 3) in the sample and a hemagglutinin produced by a microorganism belonging to the genus Conidiobolus (CA) and measuring said band 3 glycoprotein contained in a complex produced by the reaction. Because band 3 can be detected specifically, at high sensitivity, and stably by the use of CA, the method ensures qualitative or quantitative, and accurate detection of human blood component in feces or contents of digestive organs, of which the determination of the presence or quantity of human blood component by hemoglobin is difficult.

Un procédé de détection de constituants sanguins dans un échantillon consiste à faire réagir une glycoprotéine de bande 3 de membrane d'erythrocyte humain avec une hémagglutinine (CA) produite par un microbe du genre <u>conidiobolus</u> et à détecter la glycoprotéine de bande 3 contenue dans le complexe ainsi obtenu. Ce procédé peut détecter la glycoprotéine de bande 3 de façon spécifique et stable avec une grande sensibilité en utilisant la CA. Par conséquent, il permet une détection qualitative et quantitative de grande précision des constituants du sang humain présents dans les fèces ou le contenu du système digestif, milieux dans lesquels il est difficile de reconnaître la présence et le cas échéant la quantité de constituants sanguins en utilisant l'hémoglobine.

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