Method of detecting cellular immunity and application...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) A61K 38/08 (2006.01) C12Q 1/04 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/554 (2006.01) G01N 33/564 (2006.01)

Patent

CA 2476995

It is intended to provide a convenient immunity monitoring system whereby T cell frequencies specific to a plural number of antigen peptides can be assayed by using a relatively small amount of blood. Peripheral monocytes are collected and frequently stimulated with an antigen without directly using any antigen presenting cells. Then T cells specific to the antigen in the thus stimulated peripheral monocytes are detected to thereby detect antigen- specific T cells. Thus, diseases such as cancer can be prevented or treated with the use of a peptide having such a function, in particular, cancer tumor- rejection antigen peptide.

L'invention porte sur un système de contrôle approprié de l'immunité selon lequel les fréquences des lymphocytes T propres à plusieurs peptides d'antigènes peuvent être testées au moyen d'une quantité relativement faible de sang. Les monocytes périphériques sont recueillis et stimulés fréquemment par un antigène sans utiliser directement des cellules présentant des antigènes. Les lymphocytes T propres à l'antigène dans les monocytes périphériques ainsi stimulés sont détectés de façon à détecter des lymphocytes T spécifiques des antigènes. Il est ainsi possible de prévenir ou traiter des maladies telles que le cancer au moyen d'un peptide ayant une telle fonction, notamment, un peptide d'antigène empêchant la formation de tumeurs cancéreuses.

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