Method of detecting colon cancer marker

Details Method of detecting colon cancer marker Method of detecting colon cancer marker

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 1/42 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2518933

It is intended to provide a non-invasive and convenient method of detecting a tumor marker for diagnosing colon cancer which is superior in sensitivity and specificity to the existing fecal occult blood test. More specifically speaking, a method of detecting a tumor marker of colon cancer which comprises collecting biological sample which is immediately frozen using liquid nitrogen in some cases, homogenizing the sample in the presence of an RNA digesting enzyme to give a suspension, extracting RNA from the obtained suspension, subjecting the extracted RNA to reverse transcription to give cDNA, amplifying the obtained cDNA and then detecting the thus amplified cDNA. This method is characterized by involving no procedure of separating cell components from the biological sample.

L'invention concerne un procédé non invasif et pratique permettant de détecter un marqueur de tumeur pour diagnostiquer un cancer du côlon qui est supérieur au niveau de la sensibilité et de la spécificité comparé au test de sang occulte fécal courant. Plus précisément, l'invention concerne un procédé permettant de détecter un marqueur de tumeur du cancer du côlon qui consiste à prélever un échantillon biologique qui est immédiatement congelé à l'aide de l'azote liquide dans certains cas, à homogénéiser l'échantillon en présence d'un enzyme digérant des ARN pour produire une suspension, à extraire les ARN de la suspension obtenue, à soumettre les ARN extraits à une transcription inverse pour produire des ADNc, à amplifier les ADNc obtenus puis à détecter les ADNc ainsi amplifiés. Ledit procédé n'implique aucune procédure de séparation de composants cellulaires de l'échantillon biologique.

Affiliated with

Also associated with

Rating

Method of detecting colon cancer marker does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of detecting colon cancer marker, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting colon cancer marker will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1431889