C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 19/00 (2006.01) C12N 7/01 (2006.01) C12N 9/96 (2006.01) C12N 15/73 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2192662
Disclosed is an effective lambdoid bacteriophage which includes a protein construct comprising a genetically modified major tail protein truncated at its carboxy terminus, and a target molecule peptide bonded to the carboxy terminus of the tail protein. Also disclosed are nucleic acids encoding the construct and methods of detecting a molecule-of-interest in a solution and of detecting a cell which produces a molecule-of-interest.
L'invention concerne des bactériophages infectieux lambdoïques qui comprennent un produit d'assemblage protéique comprenant une protéine principale de queue, modifiée génétiquement et raccourcie à l'extrémité carboxy, et une molécule peptidique cible liée à l'extrémité carboxy de la protéine de queue. L'invention concerne également des acides nucléiques codant le produit d'assemblage, ainsi que des procédés pour détecter une molécule étudiée dans une solution et pour détecter une cellule qui produit une molécule étudiée.
Crea Roberto
Lin Edmund C. C.
Ray Bryan L.
Mbm & Co.
Symbiotech Inc.
The President And Fellows Of Harvard College
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