Method of detecting, isolating and purifying clostridium...

Details Method of detecting, isolating and purifying clostridium... Method of detecting, isolating and purifying clostridium...

G - Physics

01

N

167/44, 150/16.1

G01N 33/569 (2006.01) C12Q 1/10 (2006.01) G01N 33/566 (2006.01)

Patent

CA 1275379

METHOD OF DETECTING, ISOLATING AND PURIFYING CLOSTRIDIUM DIFFICILE TOXIN A AND ITS RECEPTORS Abstract A method is provided for detecting the presence of C. difficile toxin A. Stool or other appropriate specimen is contacted with a reagent containing an available non- reducing galactose-alpha-1-3-galactosyl structure, which is a specific receptor for toxin A. The reagent may be intact cells, cell membranes, membrane fractions containing the toxin A receptor structure, glycoconjugates, as well as the purified toxin A receptor per se. Binding of toxin A is determined by conventional assay techniques. The method may also be used to isolate and purify toxin A. Conversely, immobilized toxin A is used to detect, isolate, or purify biological materials of interest containing the receptor structure.

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