Method of detecting mutations in the gene encoding...

Details Method of detecting mutations in the gene encoding... Method of detecting mutations in the gene encoding...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2571823

The present invention describes a method for the simultaneous identification of two or more mutations located in the gene encoding Cytochrome P450-2D6. Multiplex detection is accomplished using multiplexed tagged allele specific primer extension (ASPE) and hybridization of such extended primers to a probe, preferably an addressable anti-tagged support.

L'invention porte sur un procédé qui permet l'identification simultanée d'au moins deux mutations dans le gène codant le cytochrome P450-2D6. Selon l'invention, on procède à une détection multiplexée par une extension d'amorce allèle-spécifique ("allele specific primer extension" ou ASPE) marquée multiplexée et une hybridation des amorces ainsi étendues à une sonde, de préférence un support adressable non marqué.

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