Method of detecting mutations in the gene encoding...

Details Method of detecting mutations in the gene encoding... Method of detecting mutations in the gene encoding...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2575422

The present invention describes a method for the simultaneous identification of two or more mutations located in the gene encoding Cytochrome P450-2C19. Multiplex detection is accomplished using multiplexed tagged allele specific primer extension (ASPE) and hybridization of such extended primers to a probe, preferably an addressable anti-tagged support.

La présente invention concerne une méthode permettant d'identifier simultanément au moins deux mutations situées dans le gène codant pour le cytochrome P450-2C19. Cette méthode de détection multiplex consiste à utiliser une extension d'amorce allèle-spécifique maquée, multiplexée, et à associer par hybridation les amorces ainsi étendues à une sonde, de préférence un support non marqué adressable.

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