Method of detecting nucleic acid

Details Method of detecting nucleic acid Method of detecting nucleic acid

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2169166

A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine to which a label capable of generating a detectable signal or a precursor of a label is bound, to form a complex between said nucleic acid and said polyamine, if a precursor is used, converting the precursor into said label, removing polyamine which has not formed a complex before or after the conversion of said precursor and detecting said label.

Procédé de détection d'un acide nucléique, dans lequel un support solide soupçonné de contenir un acide nucléique est mis en contact avec une polyamine à laquelle est lié un marqueur capable de produire un signal détectable ou un précurseur de celui-ci, afin de former un complexe entre un acide nucléique, s'il y en a un, et la polyamine. La polyamine qui n'a pas formé de complexe avant ou après la conversion en marqueur du précurseur éventuellement utilisé est ensuite éliminée, et le marqueur est détecté.

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