Method of detecting nucleotide sequences

Details Method of detecting nucleotide sequences Method of detecting nucleotide sequences

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1323592

PH. 34673 ABSTRACT A method for detecting the presence or absence of one or more variant nucleotide sequences. The method comprises (i) contacting a nucleic acid sample with a diagnostic primer which is substantially complementary to a diagnostic portion of a target base sequence, whereby extension of the diagnostic primer on a target template under appropriate conditions is only achieved where a terminal nucleotide of the diagnostic primer is complementary to either a suspected variant nucleotide or a corresponding normal nucleotide of the target base sequence, and (ii) detecting the presence or absence of an extension product. Kits for performing diagnostic tests are also disclosed.

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