Method of detecting organopathy-inducing factors

Details Method of detecting organopathy-inducing factors Method of detecting organopathy-inducing factors

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2157345

A method for detecting an organopathy-inducing factor in a serum or plasma sample, comprising the steps: (1) cultivating a cell from a cultured cell line derived from an organ corresponding to an organ for which disorder is to be determined, in the presence of said serum or plasma sample, whereby reactive oxygen species are released into a culture medium; (2) adding a spin-trap agent to said culture medium obtained from step (1); and (3) measuring a concentration of radicals in said culture medium obtained from step (2) is disclosed. An organopathy can be detected at an early stage.

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