Method of detecting pathogens

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2629564

A method for detecting pathogens, particularly organisms associated with sexually transmitted diseases, especially Human papilloma virus genotypes is described. The method involves the use of real-time PCR using specially designed probes. The probes, kits for carrying out the method, and methods for designing primers suitable for use in the method of the invention are also described.

La présente invention concerne un procédé destiné à la détection d~agents pathogènes, en particulier d~organismes associés à des maladies sexuellement transmissibles, plus spécifiquement les génotypes de papillomavirus humains. Le procédé utilise une amplification en chaîne par polymérase (PCR) en temps réel employant des sondes spécialement conçues. L~invention se rapporte également aux sondes, kits permettant de mettre en AEuvre le procédé et aux procédés servant à désigner les amorces à utiliser dans le procédé de l~invention.

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