C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/37 (2006.01) C12Q 1/00 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2286458
Methods of enhancing sensitivity and specificity of an assay measuring enzymatic activity in a sample by measuring enzymatic activity in the sample in the presence and absence of a specific inhibitor of the enzymatic activity are provided. Methods of measuring carboxypeptidase A levels and total carboxypeptidase A levels, wherein procarboxypeptidase A is converted to carboxypeptidase A by addition of clostripain, in a biological fluid with a carboxypeptidase A substrate, specificity of which is enhanced by addition of a carboxypeptidase A specific inhibitor are also provided. In addition, methods of diagnosing acute pancreatitis by measurement of carboxypeptidase A levels and pancreatic cancer by measurement of total carboxypeptidase A levels are also provided.
On décrit des méthodes qui permettent d'améliorer la sensibilité et la spécificité d'un dosage destiné à mesurer l'activité enzymatique dans un échantillon, en mesurant cette activité enzymatique dans l'échantillon en présence et en l'absence d'un inhibiteur spécifique de ladite activité. On décrit en outre des méthodes pour mesurer des niveaux de carboxypeptidase A et des niveaux totaux de carboxypeptidase A, le procarboxypeptidase A étant transformé en carboxypeptidase A par addition de clostripain dans un fluide biologique contenant un substrat de carboxypeptidase A dont la spécificité est améliorée par addition d'un inhibiteur spécifique de la carboxypeptidase A. On décrit enfin des méthodes permettant de diagnostiquer une pancréatite aiguë en mesurant des niveaux de carboxypeptidase A, et le cancer pancréatique en mesurant des niveaux totaux de carboxypeptidase A.
Borden Ladner Gervais Llp
Gilvarg Charles
Princeton University
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