Method of detecting shikimic acid

Details Method of detecting shikimic acid Method of detecting shikimic acid

G - Physics

01

N

G01N 33/52 (2006.01) G01N 31/22 (2006.01)

Patent

CA 2362977

The invention provides methods of detecting shikimic acid comprising the steps of: oxidizing shikimic acid with periodic acid or a reagent comprising periodate and periodic acid; adding a strong base to generate a chromophore; stabilizing the chromophore with sulfite; detecting the presence of the chromophore; and optionally quantifying the amount of the chromophore. The methods of the invention can be performed directly with tissue extracts, preferably plant tissue extracts.

L'invention concerne des procédés de détection de l'acide shikimique comprenant les étapes consistant à oxyder de l'acide shikimique avec un acide périodique ou un réactif contenant du périodate et de l'acide périodique, à ajouter une base forte pour produire un chromophore, à stabiliser le chromophore avec du sulfite, à détecter la présence du chromophore et facultativement à quantifier la quantité du chromophore. Les procédés de l'invention peuvent être mis en oeuvre directement avec des extraits de tissus, de préférence des extraits de tissus de plantes.

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