Method of detecting solid cancer cells and tissue atypia and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2224970

A method of detecting solid cancer cells which comprises measuring the expression level of a WT1 gene in a sample tissue; a method of detecting histological heterotypia which comprises measuring the expression level of a WT1 gene in a sample tissue; and a method of examining a tissue for bone marrow transplantation and peripheral blood stem cell transplantation which comprises measuring the expression level of a WT1 gene in the CD34- cell fraction of the tissue to be transplanted to thereby detect leukemic cells and solid cancer cells present therein.

Procédé de détection de cellules solides cancéreuses, qui consiste à mesurer le niveau d'expression d'un gène de WT1 dans un spécimen de tissu. Procédé de détection d'une hétérotypie histologique, qui consiste à mesurer le niveau d'expression d'un gène de WT1 dans un spécimen de tissu. Procédé d'examen d'un tissu dans le but d'effectuer une transplantation de moelle osseuse et une transplantation de cellules souches du sang périphérique, ledit procédé consistant à mesurer le niveau d'expression d'un gène de WT1 dans la fraction de cellule CD34?-¿ du tissu à transplanter, ce qui permet de détecter les cellules leucémiques et les cellules solides cancéreuses présentes dans ce tissu.

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