Method of detecting sub-ppb levels of oligonucleotides in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2180723

Disclosed is a method of detecting a single-stranded target oligonucleotide in a biological fluid. In this method, a biological fluid sample to-be-tested is contacted with helper and primer oligonucleotides, thereby forming a labelled; double-stranded molecule if the sample contains an oligonucleotide which is complementary to the target nucleotide sequences of the primer and the helper oligonucleotides. The primer so annealed to the target oligonucleotide is then ligated to the helper annealed to the target oligonucleotide. The presence of the ligation product is being indicative of the presence of the target oligonucleotide in the biological fluid.

Procédé de détection d'un oligonucléotide cible à brin unique dans un fluide biologique. Dans ce procédé, on met en contact un échantillon de fluide biologique à tester avec des oligonucléotides auxiliaire et initiateur, ceci formant une molécule marquée à deux brins si l'échantillon contient un oligonucléotide qui est complémentaire des séquences nucléotidiques des oligonucléotides initiateur et auxiliaire. L'initiateur ainsi fusionné à l'oligonucléotide cible est ensuite soudé à l'auxiliaire fusionné à l'oligonucléotide cible. La présence du produit de la ligature indique la présence de l'oligonucléotide cible dans le fluide biologique.

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