Method of detecting surface contamination by an analyte

Details Method of detecting surface contamination by an analyte Method of detecting surface contamination by an analyte

G - Physics

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G01N 33/558 (2006.01) C07D 451/02 (2006.01) C07D 489/02 (2006.01) G01N 1/02 (2006.01) G01N 33/94 (2006.01) G01N 1/00 (2006.01)

Patent

CA 2154620

Subject matter of the invention is a method for detecting surface contamination by an analyte by wiping the analyte off the surface with the aid of a wiping surface, eluting the analyte from the wiping surface with an eluant, and detecting the analyte in the eluate in an immunological detection reaction, characterized in that: a) the surface to be tested for the analyte is wiped with a wiping surface, b) the wiping surface is brought into contact with the planar surface of a capillary active, chromatographic test strip which has an eluant application zone at its one end and a target zone at its other end whereby contact is made in an area between these two zones, c) eluting liquid is applied onto the zone provided for this purpose, said liquid moving toward the target zone passes the contact site with the wiping surface as a consequence of capillary forces, whereby analyte is taken up by the eluant, and d) in the target zone, the analyte is measured in an immunological binding reaction. The method is particularly suitable for the detection of drugs on surfaces.

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