Method of detection and/or titration in vitro of an...

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2719152

The invention relates to an in vitro method for detection and/or titration in vitro of an unconventional transmissible agent (UTA) or of a pathological conformational protein, a marker of infectivity linked to the UTA, in a sample, comprising: the replication or propagation, in cells in culture, of the UTA present in the sample, and then the repeated incubation with a substrate allowing the amplification of the UTA or of the pathological conformational protein, a non-pathological conformer of the UTA, before the determination of the presence and/or of the quantity of the UTA or of the pathological conformational protein in the sample.

L'invention concerne une méthode in vitro de détection et/ou titrage in vitro d'un agent transmissible non conventionnel (ATNC) ou d'une protéine de conformation pathologique, marqueur de l'infectiosité liée à l'ATNC, dans un échantillon, comprenant : la réplication ou la propagation dans des cellules en culture de l'ATNC présent dans l'échantillon, puis l'incubation répétée avec un substrat permettant l'amplification de l'ATNC ou de la protéine de conformation pathologique, conformère non-pathologique de l'ATNC, avant la détermination de la présence et/ou de la quantité de l'ATNC ou de la protéine de conformation pathologique dans l'échantillon.

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