Method of detection of microorganisms with enhanced...

G - Physics – 01 – N

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G01N 33/569 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2690809

A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacte-riophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism. A substance which enhances bacteriophage amplification (fatty acid, eg. lauric acid) or bacteriophage sensitivity to said target microorganism (eg. pyruvate) is added.

L'invention concerne un procédé pour déterminer la présence ou l'absence d'un micro-organisme cible dans un échantillon à tester, le procédé comprenant les étapes consistant à : combiner avec l'échantillon une quantité de bactériophage capable de se fixer au micro-organisme cible pour créer un échantillon exposé au bactériophage, et une substance qui améliore l'amplification de bactériophage ou la sensibilité au bactériophage; fournir à l'échantillon exposé au bactériophage des conditions qui soient suffisantes pour permettre au bactériophage d'infecter le micro-organisme; et analyser l'échantillon exposé au bactériophage pour détecter la présence ou l'absence d'un marqueur de bactériophage pour déterminer la présence ou l'absence du micro-organisme cible.

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