Method of determining 1,5-anhydroglucitol, and reagent...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/04 (2006.01) C12Q 1/32 (2006.01) C12Q 1/54 (2006.01)

Patent

CA 2655890

A method for the determination of 1,5-anhydroglucitol, characterized by using any one of the following proteins (a) to (c): (a) a protein which comprises an amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence having the deletion, substitution and/or addition of one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity; and (c) a protein which comprises an amino acid sequence having at least 60% sequence homology to the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity.

L'invention concerne un procédé pour le dosage du 1,5-anhydroglucitol, caractérisé par l'utilisation de l'une quelconque des protéines (a) à (c) suivantes : (a) une protéine qui comprend une séquence d'acides aminés représentée dans SEQ ID NO:2; (b) une protéine qui comprend une séquence d'acides aminés ayant la délétion, substitution et/ou addition d'un ou plusieurs restes d'acides aminés dans la séquence d'acides aminés représentée dans SEQ ID NO:2 et qui a une activité sorbose déshydrogénase; et (c) une protéine qui comprend une séquence d'acides aminés ayant au moins 60% d'homologie de séquence à la séquence d'acides aminés représentée dans SEQ ID NO:2 et qui a une activité sorbose déshydrogénase.

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