Method of determining antigenically active amino acid sequences

Details Method of determining antigenically active amino acid sequences Method of determining antigenically active amino acid sequences

G - Physics

01

N

167/37, 167/44

G01N 33/566 (2006.01) C12Q 1/28 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1220420

ABSTRACT OF THE DISCLOSURE A method of detecting or determining a sequence of amino acids which is antigenically active within a known amino acid sequence of a protein or portion thereof comprises the steps of: 1. synthesising a plurality of peptides, each of said peptides comprising a sequence of a plurality of amino acids which corresponds to a sequence within the known amino acid sequence, and the said peptides having overlapping amino acid sequences; 2. contacting each of said peptides with antibody against the protein or portion of interest; and 3. detecting or determining the presence or absence of an antigen-antibody reaction between each of said peptides and said antibody to indicate whether or not said peptide has antigenic activity.

449141

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