Method of determining cholinesterase activity and choline...

C - Chemistry – Metallurgy – 12 – Q

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260/476.1, 150/1

C12Q 1/28 (2006.01) C12Q 1/46 (2006.01)

Patent

CA 1137508

ABSTRACT Cholinesterase activity is determined by means of the choline and/or acid obtained by the action of cholinesterase on a compound of the formula:- Image I [wherein A represents a hydrogen atom or a C1-5 alkyl or alkoxy group and a represents a group of the formula -R-COO- (in which - R - represents an alkenyl group with 2 to 6 carbon atoms); or A represents a hydroxy group in either or both o - positions of the benzene ring or a C1-5 alkyl group and B represents a group of the formula - COO - ; X represents a stabilising anion and n represents the charge on the anion]. The method of the invention ensures a high resistance to saturation, good stability of the compounds used as substrates, a conveniently low reaction rate and a high degree of accuracy.

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