Method of determining epidermal growth factor receptor and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2436910

The present invention relates to prognostic methods which are useful in medicine, particularly cancer chemotherapy. The object of the invention to provide a method for assessing HER2-neu and/or EGFR expression levels in fixed or fixed and paraffin embedded tissues and prognosticate the probable sensitivity of a patient's tumor to treatment with receptor tyrosine kinase targeted chemotherapy by examination of the amount of HER2-neu and/or EGFR mRNA in a patient's tumor cells and comparing it to a predetermined threshold expression level for those genes. More specifically, the invention provides to oligonucleotide primer pairs EGFR and HER2-neu and methods comprising their use for detecting levels of EGFR and HER2-neumRNA, respectively.

L'invention concerne des méthodes de pronostic utiles en médecine, en particulier pour les chimiothérapies anticancéreuses. Cette invention a pour objet la mise au point d'une méthode permettant d'évaluer les niveaux d'expression de HER2-neu et/ou deEGFR dans des tissus fixés ou fixés et enrobés de paraffine, et de pronostiquer la sensibilité probable de la tumeur du patient à un traitement de chimiothérapie ciblé sur les récepteurs à activité tyrosine kinase, en examinant les quantités de HER2-neu et/ou d'ARNm de EGFR dans les cellules tumorales d'un patient, et en comparant les valeurs mesurées à un niveau d'expression seuil prédéterminé pour ces gènes. De manière plus spécifique, cette invention concerne des paires d'amorces d'oligonucléotides EGFR et HER2-neu et des méthodes consistant à utiliser celles-ci pour détecter les niveaux respectifs de EGFR et d'ARNm de HER2-neu .

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