Method of determining the amount of cholesterol in a...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/60 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2162286

Provided are a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises measuring the amount of cholesterol in low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and chylomicron (CM) in a sample in the presence of a sugar compound and/or a protein solubilizing agent, and calculating the difference between the amount of cholesterol in LDL, VLDL and CM and the total amount of cholesterol in the sample, and a method of determining the amount of cholesterol in HDL, which comprises measuring the amount of cholesterol in HDL in a sample in the presence of a sugar compound and/or a protein solubilizing agent.

La présente invention concerne un procédé de recherche du cholestérol dans les lipoprotéines haute densité (HDL). Ce procédé consiste à mesurer la teneur en cholestérol dans chacune des lipoprotéines basse densité (LDL) des lipoprotéines très basse densité (VLDL) et des chylomicrons (CM) d'un échantillon en présence d'un composé de sucre et/ou d'un agent solubilisant des protéines. Le procédé consiste ensuite à calculer la différence entre la teneur ainsi obtenue et la teneur du cholestérol total dans l'échantillon. L'invention concerne également un procédé de recherche du cholestérol dans un HDL. Ce dernier procédé consiste à mesurer de la teneur en cholestérol d'un échantillon de HDL en présence d'un composé de sucre et/ou d'un agent solubilisant des protéines.

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