Method of determining the diversity of t lymphocytes in a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2596389

The invention relates to the field comprising the diagnosis of possible immune system disorders and the analysis of immune responses. In particular, the invention relates to a method of determining the diversity of T lymphocytes in a biological sample, based on the molecular analysis of the structure of the junctions resulting from the recombination rearrangement V(D)J of element .delta.Rec-1 with an AJ gene. More specifically, the invention relates to a method of analysing the combinatorial diversity and/or the junctional diversity of the excision circles (TREC) resulting from the rearrangement of .delta.Rec-1, in order to determine the heterogeneity of a given population of T lymphocytes.

La présente invention concerne le domaine du diagnostic d'éventuels désordres du système immunitaire et l'analyse des réponses immunitaires. L'invention porte en particulier sur un procédé de détermination de la diversité des lymphocytes T dans un échantillon biologique, basé sur l'analyse, au niveau moléculaire, de la structure des jonctions résultant du réarrangement par recombinaison V(D)J de l'élément dRec-1 avec un gène AJ. Plus spécifiquement, la présente invention propose d'analyser la diversité combinatoire et/ou la diversité jonctionnelle des cercles d'excision (TREC) résultant du réarrangement de dRec-1, afin de déterminer l'hétérogénéité d'une population donnée de lymphocytes T.

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