Method of determining the probability of a therapeutic...

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/48 (2006.01) G01N 33/573 (2006.01) G01N 33/574 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2705272

A prognostic assay and kit and method of use thereof are provided. The kit and assay are used to determine the likelihood of a diseased cell or tissue having a therapeutic response to treatment with a cardiac glycoside in a disease having an etiology associated with excessive cell proliferation. The kit and assay are used to determine the ratio of isoforms of the a subunit of Na, K-ATPase obtained from the diseased cell or tissue. The kit can be used to predict the therapeutic responsiveness of cancer or tumor in a subject to treatment with a cardiac glycoside. The kit and assay can be incorporated in a method of treating a disease or disorder having an etiology associated with excessive cell proliferation with a composition comprising a cardiac glycoside.

L'invention concerne un test et un kit de pronostic ainsi que leur méthode d'utilisation. Le kit et le test sont utilisés pour déterminer la probabilité qu'une cellule ou un tissu malade présente une réponse thérapeutique au traitement avec un glycoside cardiaque dans le cas d'une maladie dont l'étiologie est associée à une prolifération cellulaire excessive. Le kit et le test sont utilisés pour déterminer le ratio des isoformes de la sous-unité a de la Na/K-ATPase provenant de la cellule ou du tissu malade. Le kit peut être utilisé pour prédire chez un sujet la capacité de réponse thérapeutique d'un cancer ou d'une tumeur au traitement avec un glycoside cardiaque. Le kit et le test peuvent être incorporés dans une méthode consistant à traiter une maladie ou un trouble dont l'étiologie est associée à une prolifération cellulaire excessive avec une composition comprenant un glycoside cardiaque.

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