Method of determining the sequence of nucleic acids...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01J 19/00 (2006.01) G01N 21/64 (2006.01) G08C 17/00 (2006.01)

Patent

CA 2238645

A method is described for determining the sequence of nucleic acids. The method employs small solid phase particles having transponders, with a primary layer of an oligonucleotide of known sequence attached to the outer surface of the particle. A read/write scanner device is used to encode and decode data on the transponder. The stored data includes the sequence of the oligonucleotide immobilized on the transponder. The sequence of sample nucleic acids is determined by detecting annealing to an oligonucleotide bound to a particle, followed by decoding the transponder to determine the sequence of the oligonucleotide.

Méthode de détermination de la séquence d'acides nucléiques. On utilise de petites particules en phase solide munies de transpondeurs, avec une couche primaire constituée d'un oligonucléotide de séquence connue fixé à la surface extérieure de la particule. On utilise un scanner de lecture/écriture pour coder et décoder les données sur le transpondeur. Les données enregistrées sont notamment la séquence de l'oligonucléotide immobilisé sur le transpondeur. On détermine la séquence des acides nucléiques faisant l'objet de l'analyse en détectant la renaturation à un oligonucléotide lié à une particule, puis en décodant le transpondeur pour déterminer la séquence de l'oligonucléotide.

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