Method of determining the total fibrinolytic activity in the...

C - Chemistry – Metallurgy – 12 – Q

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167/114, 150/15.

C12Q 1/37 (2006.01) A61K 35/16 (2006.01) C12Q 1/56 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2007296

Method of determining fibrinolytic activity in the plasma Abstract The method is characterised in that a) a quantity of fibrin sufficient to produce turbidity is added to a dilute platelet poor plasma sample or is produced in situ and the time-dependent change in turbidity or the time-dependent formation of fibrin cleavage products is measured or b) a chromogenic plasmin substrate and a quantity of fibrin, fibrinogen cleavage products or an enzyme which produces fibrin in situ, insufficient to produce turbidity, is added to a dilute platelet poor plasma sample and the time-dependent colour formation is measured. Polyethyleneglycol (Mol.Wt range 8,000 to 35,000) may be present in the diluted sample to increase opacity.

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