Method of diagnosing infection

Details Method of diagnosing infection Method of diagnosing infection

G - Physics

01

N

167/43

G01N 33/569 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1233746

ABSTRACT OF THE DISCLOSURE A method of serologically diagnosing bovine leukemia virus infection in cattle. The method includes pro- viding a test plate having a plurality of wells. At least some of the wells are coated with a bovine leukemia virus antigen. An ELISA buffer including a blocking agent to resist nonspecific binding is introduced into the wells. The test sera is introduced into the wells either directly or by premixing with the blocking solution. An enzyme analyte is introduced into the wells and subsequently a substrate- chromagen is introduced into the wells. The reaction is read or stopped. By visual determination based upon the color of the wells, either with the naked eye or with the aid of appropriate apparatus, a determination is made. Appropriate control wells may be provided. Intermediate washing and incubation stages are employed. Among the pre- ferred blocking agents are those selected from the group consisting of goat serum, chicken serum, guinea pig and sheep serum.

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