Method of diagnosis of infection by mycobacteria and...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/04 (2006.01) A61K 39/395 (2006.01) A61P 31/06 (2006.01) C07K 14/35 (2006.01) C07K 16/12 (2006.01) C07K 16/40 (2006.01) C07K 17/00 (2006.01) C07K 19/00 (2006.01) C12N 11/00 (2006.01) G01N 33/543 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2725613

The present invention provides isolated M tuberculosis protein that is a putative Ketol-acid reductoisomerase (KARI, SEQ ID NO 1) and immunogenic peptide fragments thereof, and antibodies produced against the full-length protein and immunogenic peptide fragments for the diagnosis of tuberculosis and/or infection by one or more mycobacteria of the M tubercu-losis complex in humans, for example using an antigen-based sandwich ELISA format The present invention also provides multi-analyte assays in which the KARI-based diagnostic assays of the present invention are multiplexed with the detection of one or more immunogenic epitopes from one or more other proteins of said mycobacteria e g, any one of SEQ IDS NOs 2, 14, 21, 28-29, 36, or 44, including any combinations thereof

La présente invention porte sur une protéine de M. tuberculosis isolée qui est une cétol-acide réductoisomérase putative (KARI; SEQ ID NO: 1) et sur des fragments peptidiques immunogènes de celle-ci, et sur des anticorps produits contre la protéine de longueur entière et les fragments peptidiques immunogènes pour le diagnostic de la tuberculose et/ou d'une infection par une ou plusieurs mycobactéries du complexe de M. tuberculosis chez les êtres humains, par exemple à l'aide d'un format ELISA en sandwich à base d'antigène. La présente invention porte également sur des dosages à multiples analytes dans lesquels les dosages de diagnostic à base de KARI de la présente invention sont multiplexés avec la détection d'un ou plusieurs épitopes immunogènes provenant d'une ou plusieurs autres protéines desdites mycobactéries, par exemple, l'une quelconque des SEQ IDS NOs: 2, 14, 21, 28-29, 36 ou 44, comprenant toutes combinaisons de celles-ci.

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